New approach to modulate retinal cellular toxic effects of high glucose using marine epa and dha

نویسندگان

  • Mélody Dutot
  • Violaine de la Tourrette
  • Roxane Fagon
  • Patrice Rat
چکیده

BACKGROUND Protective effects of omega-3 fatty acids against cellular damages of high glucose were studied on retinal pigmented epithelial (RPE) cells. METHODS Retinal epithelial cells were incubated with omega-3 marine oils rich in EPA and DHA and then with high glucose (25 mM) for 48 hours. Cellular responses were compared to normal glucose (5 mM): intracellular redox status, reactive oxygen species (ROS), mitochondrial succinate deshydrogenase activity, inflammatory cytokines release and caveolin-1 expression were evaluated using microplate cytometry, ELISA and flow cytometry techniques. Fatty acids incorporation in retinal cell membranes was analysed using chromatography. RESULTS Preincubation of the cells with fish oil decreased ROS overproduction, mitochondrial alterations and TNFα release. These protective effects could be attributed to an increase in caveolin-1 expression induced by marine oil. CONCLUSION Marine formulations rich in omega-3 fatty acids represent a promising therapeutic approach for diabetic retinopathy.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of enriched Artemia urmiana with HUFA on growth, survival, and fatty acids composition of the Persian sturgeon larvae (Acipenser persicus)

 Recently, the nutritional requirements of marine finfish larvae have received considerable attention, and studies have shown that docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) and arachidonic acid (ARA) affects the growth and survival of marine finfish larvae. This study investigated the effects of different Artemia enrichments containing variable amounts of DHA and EPA on the gro...

متن کامل

Effects of enriched Artemia urmiana with HUFA on growth, survival, and fatty acids composition of the Persian sturgeon larvae (Acipenser persicus)

 Recently, the nutritional requirements of marine finfish larvae have received considerable attention, and studies have shown that docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) and arachidonic acid (ARA) affects the growth and survival of marine finfish larvae. This study investigated the effects of different Artemia enrichments containing variable amounts of DHA and EPA on the gro...

متن کامل

Squid (Loligo loligo ): The new source to extract omega-3 and omega-6 rich marine oils

Squids belong to the cephalopod family, which composes more than 5% of the total annual fishing volume of southern Iran as by- products; however, these do not have significant usage in Iran. This product was studied to extract the high-value products, i.e. marine oils rich in unsaturated multiple-band fatty acids from 50kg squid (Loligo loligo) obtained from Chabahar fishing port and transferre...

متن کامل

Purified eicosapentaenoic and docosahexaenoic acids have differential effects on serum lipids and lipoproteins, LDL particle size, glucose, and insulin in mildly hyperlipidemic men.

BACKGROUND Regular consumption of n-3 fatty acids of marine origin can improve serum lipids and reduce cardiovascular risk. OBJECTIVE This study aimed to determine whether eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have differential effects on serum lipids and lipoproteins, glucose, and insulin in humans. DESIGN In a double-blind, placebo-controlled trial of parallel design, 59 ...

متن کامل

Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro

Methylmercury (MeHg) is a ubiquitous environmental contaminant which bioaccumulates in marine biota. Fish constitute an important part of a balanced human diet contributing with health beneficial nutrients but may also contain contaminants such as MeHg. Interactions between the marine n-3 fatty acids eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) with MeHg-induced ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2011